Plasmodium falciparum is exposed to endogenous and exogenous oxidative stress after invading erythrocytes. To maintain redox homeostasis and ensure survival, the parasite owns a complex antioxidant defence network consisting primarily of the glutathione and thioredoxin systems. The P. falciparum genome encodes homologues of most proteins involved in these pathways, highlighting their biological importance and suggesting that they may represent promising targets for antimalarial therapies. Using a combination of genetic tools, I want to identify which components of the parasite’s redox network are essential for cell functions and for overall survival. To achieve this, I combine CRISPR/Cas9-mediated genome editing with the DiCre conditional knockout system. Following conditional gene disruption, I investigate the effects on redox-dependent processes, redox homeostasis, parasite growth, and overall viability. If required, I can also generate double-knockout parasite lines to examine potential overlaps or synergistic interactions between different redox proteins. In the long term, I hope to contribute to a systematic understanding of the redox system in P. falciparum and its potential as a therapeutic target.